Coding
Part:BBa_K3505041:Design
Designed by: Venetios Michelioudakis Group: iGEM20_Thessaly (2020-10-23)
LacZa for Bacterial GoldenBraid plasmids
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 366
Illegal PstI site found at 405 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 366
Illegal BamHI site found at 387 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7
Illegal BsaI.rc site found at 616
Design Notes
The Judging form didn't let us to subbmit the whole plasmids for the Best Part Collection, because the whole plasmid had inside the restriction sites that made it not combatilbe for some Assembly. So we had to subbmit separatelly the backbone and the insert.